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3D LUNG NAVIGATION SYSTEM is a software application which is used with a bronchoscopy unit. By providing patient's Computerized Tomography (CT) images and bronchoscopy camera feed, it is able to localise target lesion and support early diagnosis of suspicious lesions with almost 100% accuracy. In order to detect and take samples from a target lesion our software transforms CT images into virtual 3D airway and blood vessel volumes and our system asks operator to mark the lesion on user interface. Then the system calculates the safest route to lesion to eliminate risks of bleeding inside lungs. Calculated route will be shown on both virtual and real bronchoscopy video frames to lead operator into the lesion. At the end opearator will be able to see where the lesion is and which angle bronchoscopic needle should take sample from it.